A dna fragment of 348 - bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132 - bp by haelli . endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern - blot with spesific probe had the different cleavage pattern . the isolated 285 - bpand 348 - bp dna was ligated with plasmid puc18 . the ligation mixture was used to transform e . coli jm109and the transformants were plated on lb agar containing antibiotics . plasmid dna containing cloned genes were used for direct sequencing 提示1999年的疫情由不同的病原菌引起。另外使用針對志賀毒素2及其變種的引物對進行pcr檢測、細菌染色體pst和pcr產(chǎn)物的hae 、 ras酶切分析,以及pcr產(chǎn)物的序列分析,發(fā)現(xiàn)2000年從江蘇省徐州市患者和家畜家禽糞便標本分離的大腸桿菌o157 : h7菌株僅僅攜帶slt2vha基因。